Immunogene PCV2-Zusammensetzungen und Verfahren zur Herstellung Solcher Zusammensetzungen

Details

Veröffentlichungs-Nr.

EP2460820

Aktenzeichen

EP12155382

Anmeldetag

29. Dezember 2005

Veröffentlichung

19. Juni 2019

Erteilung

19. Juni 2019

Rechtsraum

EP

IPC

C07K14/01A61K39/12C12N7/04C12Q1/68A61K39/295

Abstract

An improved method for recovering the protein expressed by open reading frame (2) from porcine circovirus type (2) is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame (2) coding sequences into cells contained in growth media, causing the virus to express open reading frame (2), and recovering the expressed protein in the supernate. This recovery should take place beginning approximately (5) days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time consuming extraction procedures required to separate and recover the recombinant protein from within the cells.

Anmelder

Firmen

Boehringer Ingelheim Animal Health USA Inc.
Boehringer Ingelheim Vetmedica, Inc.

Land

🇺🇸 USA

Fachgebiete