Verfahren zum Nachweis von methyliertem DNA in einer Probe
Anmelder: SYSMEX CORPORATION 🇯🇵
Details
- Veröffentlichungs-Nr.
- EP2492356
- Aktenzeichen
- EP12157307
- Anmeldetag
- 28. Februar 2012
- Veröffentlichung
- 21. August 2013
- Erteilung
- 21. August 2013
- Rechtsraum
- EP
- IPC
- C12Q1/68
Abstract
In order to simply obtain a methylated DNA sample, a method including bringing a sample which may include methylated DNA into contact with a protein capable of binding to methylated DNA to bind the methylated DNA in the sample to the protein, bringing the sample obtained into contact with at least one deoxyribonuclease to degrade DNA in the sample; and detecting methylated DNA which is not degraded by the deoxyribonuclease by the binding of the sample obtained to the protein is used. In the method, at least one type of deoxyribonucleases is a deoxyribonuclease different from a methylation-sensitive restriction enzyme capable of degradating a single stranded DNA.
Anmelder
- Firma
- SYSMEX CORPORATION
- Land
- 🇯🇵 Japan
Japanisches Unternehmen mit Sitz in Kobe, spezialisiert auf Entwicklung und Herstellung von Labordiagnostik- und Analysegeräten, insbesondere für Hämatologie und klinische Chemie.
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