Verfahren und Kit zur Verstärkung und Detektion von Polynukleotid

Details

Veröffentlichungs-Nr.

EP2546362

Aktenzeichen

EP12176172

Anmeldetag

12. Juli 2012

Veröffentlichung

16. März 2016

Erteilung

16. März 2016

Rechtsraum

EP

IPC

C12Q1/68C12N9/12

Abstract

Disclosed is a method of amplifying a polynucleotide, comprising: (a) mixing primers for amplifying the polynucleotide, a polymerase, nucleotide substrates and a template polynucleotide, and (b) amplifying the polynucleotide by a polymerase reaction, wherein the polymerase has an amino acid sequence consisting of SEQ ID NO:1 or an amino acid sequence homologous to SEQ ID NO:1, and wherein the amino acid residue corresponding to position 651 of the amino acid sequence has been replaced with glutamic acid.

Anmelder

Firma

ARKRAY, Inc.

Land

🇯🇵 Japan

Fachgebiete