Verfahren und Kit zur Verstärkung und Detektion von Polynukleotid

Details

Veröffentlichungs-Nr.

EP2546363

Aktenzeichen

EP12176175

Anmeldetag

12. Juli 2012

Veröffentlichung

4. März 2015

Erteilung

4. März 2015

Rechtsraum

EP

IPC

C12Q1/68C12N9/12

Abstract

Disclosed is a method of amplifying a polynucleotide, comprising: (a) mixing primers for amplifying the polynucleotide, a polymerase, nucleotide substrates and a template polynucleotide, and (b) amplifying the polynucleotide by a polymerase reaction, wherein the polymerase has an amino acid sequence consisting of SEQ ID NO:1 or an amino acid sequence homologous to SEQ ID NO:1, and wherein the amino acid residue corresponding to position 653 of the amino acid sequence has been replaced with glutamic acid. The present inventors have discovered that Tth polymerase wherein proline at position 653 in SEQ ID NO:1 has been replaced with glutamic acid has reverse transcription activity (i.e. reverse transcriptase activity) and can incorporate deoxyuridine triphosphate.

Anmelder

Firma

ARKRAY, Inc.

Land

🇯🇵 Japan

Fachgebiete