Verfahren zur Detektion von Mutationen in den Genen IL28B (RS8099917) und ITPA (RS1127354)

Details

Veröffentlichungs-Nr.

EP2570492

Aktenzeichen

EP12184426

Anmeldetag

14. September 2012

Veröffentlichung

16. Juli 2014

Erteilung

16. Juli 2014

Rechtsraum

EP

IPC

C12Q1/68

Abstract

A probe for detecting a polymorphism in the IL28B gene, comprising the fluorescently labeled oligonucleotide P1 or P1' below: (P1) an oligonucleotide comprising a nucleotide sequence of 7 to 28 consecutive nucleotides containing nucleotides 301 to 307 in SEQ ID NO:1 or a homologous sequence thereof, wherein the nucleotide corresponding to the nucleotide at position 307 is a cytosine and is labeled with a fluorescent dye; (P1') an oligonucleotide comprising a nucleotide sequence of 7 to 28 consecutive nucleotides containing nucleotides 301 to 307 in SEQ ID NO:1 or a nucleotide sequence which hybridizes with the complementary strand of SEQ ID NO:1 under stringent conditions, wherein the nucleotide corresponding to the nucleotide at position 307 is a cytosine and is labeled with a fluorescent dye. A probe for detecting a polymorphism in the ITPA gene, comprising at least one fluorescently labeled oligonucleotide selected from P2 to P3' below: (P2) an oligonucleotide comprising a complement of a nucleotide sequence of 13 to 28 consecutive nucleotides containing nucleotides 239 to 251 in SEQ ID NO:2 or a homologous sequence thereof, wherein the nucleotide corresponding (i.e. complementary) to the nucleotide at position 239 is a cytosine and is labeled with a fluorescent dye; (P2') an oligonucleotide comprising a nucleotide sequence which hybridizes with a nucleotide sequence of 13 to 28 consecutive nucleotides containing nucleotides 239 to 251 in SEQ ID NO:2 under stringent conditions, wherein the nucleotide corresponding (i.e. complementary) to the nucleotide at position 239 is a cytosine and is labeled with a fluorescent dye; (P3) an oligonucleotide comprising a nucleotide sequence of 6 to 42 consecutive nucleotides containing nucleotides 251 to 256 in SEQ ID NO:2 or a homologous sequence thereof, wherein the nucleotide corresponding to the nucleotide at position 256 is a cytosine and is labeled with a fluorescent dye; (P3') an oligonucleotide comprising a nucleotide sequence of 6 to 42 consecutive nucleotides containing nucleotides 251 to 256 in SEQ ID NO:2 or a nucleotide sequence which hybridizes with the complementary strand of SEQ ID NO: 2 under stringent conditions, wherein the nucleotide corresponding to the nucleotide at position 256 is a cytosine and is labeled with a fluorescent dye.

Anmelder

Firma

ARKRAY, Inc.

Land

🇯🇵 Japan

Fachgebiete