Phagen-Anzeigebibliothek

Details

Veröffentlichungs-Nr.

EP2940138

Aktenzeichen

EP14166662

Anmeldetag

30. April 2014

Veröffentlichung

28. Februar 2018

Erteilung

28. Februar 2018

Rechtsraum

EP

IPC

C12N15/10C07K1/04

Abstract

A library of replicating entities, each entity comprises a recombinant vector comprising a randomized nucleic acid sequence, having the reading frame structure [NXX] n [CorAA] [NXX] m [NZZ] o , or [NZZ] o [NXX] m [CorAA] [NXX] n . Each NXX is independently a codon encoding for any amino acid except cysteine, CorAA is a codon encoding for cysteine or at least one other amino acid, each NZZ is independently a codon encoding for any amino acid, and n is an integer from 0 to 40, m is an integer from 1 to 20, o is an integer from 1 to 40, and at least 20 percent of CorAA encode for cysteine. The invention further relates to a set of recombinant vectors and to a set of randomized oligonucleotides, each oligonucleotide having said structure, as well as to a method for generating a library of replicating entities and to a method for identifying an amino acid polymer.

Anmelder

Firma

Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.

Land

🇩🇪 Deutschland

Fachgebiete