Verwendung eines Laminins zur Differenzierung von pluripotenten Zellen in Hepatocyten-Zelllinien

Details

Veröffentlichungs-Nr.

EP3059307

Aktenzeichen

EP15305265

Anmeldetag

20. Februar 2015

Veröffentlichung

4. Mai 2022

Erteilung

4. Mai 2022

Rechtsraum

EP

IPC

A01K67/027C12N5/071

Abstract

The invention relates to the use of a laminin (LN) as a matrix for hepatic differentiation. The invention also relates to a method for inducing hepatic differentiation comprising the steps of: (i) providing a population of human pluripotent cells, (ii) culturing the population on a support coated with a laminin in a endoderm induction medium to produce a population of human DE cells, (iii) culturing said population of human DE cells on a support coated with a laminin in a hepatic induction medium to produce a population of human hepatoblasts-like cells, and (iv) optionally culturing said population of human hepatoblasts-like cells on a support coated with a laminin in a hepatic maturation medium to produce a population of human fetal hepatocyte-like cells. The invention further relates to a population of human hepatoblasts-like cells or human fetal hepatocyte-like cells obtained by the method of the invention. The invention further relates to a population of human hepatoblasts-like cells expressing HNF4α and not expressing or substantially not expressing AFP for use in a method of treatment of the human body.

Anmelder

Firmen

INSERM (Institut National de la Santé et de la Recherche Médicale)
Nantes Université
Université de Nantes

Land

🇫🇷 Frankreich

Fachgebiete