Verfahren zur Analyse der Fähigkeit einer Substanz zur Durchquerung einer Doppelschichtigen Membran

Details

Veröffentlichungs-Nr.

EP3267196

Aktenzeichen

EP16305840

Anmeldetag

4. Juli 2016

Veröffentlichung

23. Oktober 2019

Erteilung

23. Oktober 2019

Rechtsraum

EP

IPC

G01N33/92G01N33/68B01L3/00

Abstract

The present invention relates to a method to analyse the ability of a substance (3) to cross a bilayer membrane comprising the following steps: (i) providing in a hydrophobic medium (1) a first droplet D 1 of an aqueous solution AS 1 , wherein the droplet D 1 is surrounded by a monolayer of amphiphilic molecules (2), (ii) providing in the said hydrophobic medium (1) a second droplet D 2 of an aqueous solution AS 2 containing the substance (3) to be analysed, wherein the droplet D 2 is surrounded by a monolayer of amphiphilic molecules (2), (iii) putting the first droplet D 1 and the second droplet D 2 into contact so as to form a bilayer of amphiphilic molecules (2) in the contact area and to have a contact angle 2¸ between the two droplets D 1 and D 2 comprised between 10° and 180°, (iv) adding to the hydrophobic medium (1) a solvent capable of separating the droplets D 1 and D 2 put into contact, (v) collecting the droplet D 1 , and (vi) analysing the content of the droplet D 1 in order to determine the presence or not of the substance (3).

Anmelder

Firmen

Paris Sciences et Lettres - Quartier Latin
Université Paris Diderot - Paris 7
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE -CNRS-
Sorbonne Université
Université Pierre et Marie Curie (Paris 6)

Land

🇫🇷 Frankreich

Fachgebiete