Systeme und Verfahren zur Identifizierung von Hla-Assoziierten Tumorpeptiden

Details

Veröffentlichungs-Nr.

EP3543699

Aktenzeichen

EP19164714

Anmeldetag

22. März 2019

Veröffentlichung

4. August 2021

Erteilung

4. August 2021

Rechtsraum

EP

IPC

G01N33/569G01N33/68

Abstract

Systems and methods for determining amino acid sequences of peptides that bind to MHC-I or HLA-I complex or MHC-II or HLA-II complex are provided. One embodiment includes isolating peptides from MHC or HLA class I or class II-peptide complexes and adding one or more known labeled peptides of interest to form a sample containing labeled peptides and unlabeled isolated peptides. The method also includes analyzing the sample with an LC-MS/MS system to obtain sequence data of the peptides, and increasing the sensitivity of the LC-MS/MS system when the labeled peptide is detected by the LC-MS/MS system. The method then concludes with determining the amino acid sequence of the unlabeled peptides in an m/z range that includes the m/z of the labeled peptide. The system can be triggered to increase the sensitivity in or near the m/z of the labeled peptide using an algorithm or computer program.

Anmelder

Firma

Regeneron Pharmaceuticals, Inc.

Land

🇺🇸 USA

Fachgebiete