System und Verfahren zum Lysieren und Entfernen Roter Blutkörperchen aus einem Zellprodukt

Details

Veröffentlichungs-Nr.

EP3845258

Aktenzeichen

EP20216919

Anmeldetag

23. Dezember 2020

Veröffentlichung

5. Februar 2025

Erteilung

5. Februar 2025

Rechtsraum

EP

IPC

A61M1/34A61M1/36C12N5/078G01N33/49G01N33/50

Abstract

A method is provided for removing red blood cells from a suspension comprising red blood cells, white blood cells, platelets and plasma using a spinning membrane separator. The method comprises: a) flowing whole blood into the gap of the spinning membrane separator; b) collecting red blood cells and white blood cells in the gap and passing plasma and platelets through the membrane; c) introducing a first quantity of lysing buffer into the gap; d) incubating the red blood cells, white blood cells and lysing buffer in the gap for a period of time to cause a lysis reaction with the red blood cells; e) introducing a second quantity of lysing buffer into the gap to displace the first quantity of lysing buffer and a first quantity of red blood cell debris out of the gap; f) introducing a first quantity of wash buffer into the gap to quench the lysis reaction and displace the second quantity of lysing buffer and a second quantity of red blood cell debris out of the gap; and g) introducing a second quantity of wash buffer into the gap to flow washed white blood cells out of the housing.

Anmelder

Firma

Fenwal, Inc.

Land

🇺🇸 USA

Fachgebiete