Verfahren und Vorrichtung zur Multiplexierten Bildgebung von Biomolekülen durch Iteratives Entmischen von Fluorophorsignalen

Details

Veröffentlichungs-Nr.

EP3961194

Aktenzeichen

EP21192162

Anmeldetag

19. August 2021

Veröffentlichung

8. November 2023

Erteilung

8. November 2023

Rechtsraum

EP

IPC

G01N21/64

Abstract

The present disclosure provides a method and apparatus for biomolecular multiplexed imaging through the iterative unmixing of fluorophores. According to the first embodiments of the present disclosure, although signals of two fluorophores are detected in the first fluorescent detection spectral range, the signals of the two images obtained from two detection spectral ranges can be unmixed through the iterative minimization of mutual information. Furthermore, the present disclosure provides a multi-color unmixing method and apparatus through the iterative minimization of mutual information. In the second embodiments of the present disclosure, a plurality of images of a plurality of fluorophores marking different biomolecules, respectively, for example, N fluorophores are obtained. Images, each containing the signals of single fluorophore can be obatined from the obtained images while minimizing the mutual information shared by images of each of pairs each consisting of two of the obtained images.

Anmelder

Firma

Korea Advanced Institute of Science and Technology

Land

🇰🇷 Südkorea

Fachgebiete