Vorrichtung zur Analyse von Einzelzellen mittels Mikroporen

Details

Veröffentlichungs-Nr.

EP4067865

Aktenzeichen

EP22164244

Anmeldetag

24. März 2022

Veröffentlichung

27. November 2024

Erteilung

27. November 2024

Rechtsraum

EP

IPC

G01N15/12

Abstract

Disclosed is a device for analyzing a single cell using a micropore (310), including an inlet chamber (100) in which a solution including a target cell (C) is contained; an outlet chamber (200) provided on an opposite side of the inlet chamber; a pore membrane (300) disposed between the inlet chamber and the outlet chamber and including a micropore (310) therein; a pressure generating means (400) provided in the inlet chamber or the outlet chamber and configured to generate a pressure difference between the inlet chamber and the outlet chamber such that a solution in the inlet chamber flows toward the outlet chamber to pass through the micropore; and a pair of electrodes (510, 520) respectively disposed in front and rear of the pore membrane and provided to be in electrical contact with the flowing solution to measure an ion conductivity change in front and rear of the pore membrane, wherein a diameter D of the micropore (310) is larger than a diameter of the target cell (C), a thickness t of the pore membrane (300) is 0.5 µm to 1mm, and a slenderness ratio (t/D) is 0.001 to 5.According to the present disclosure, a deformed cell can be rapidly and accurately detected by measuring the deformed characteristic of single cell by shear force, and the number, size, and deformability of the cell when the cell passes through a pore with a micro-sized diameter in a flow field generated by a pressure difference.

Anmelder

Firma

Korea University Research and Business Foundation

Land

🇰🇷 Südkorea

Fachgebiete