Integriertes System für Chemische, Biochemische oder Biologische Reaktionen in einer Mikroplatte unter Einwirkung eines Temperaturgradienten

Details

Veröffentlichungs-Nr.

EP4378586

Aktenzeichen

EP22210890

Anmeldetag

1. Dezember 2022

Veröffentlichung

5. Juni 2024

Rechtsraum

EP

IPC

B01L7/00

Abstract

A system for conducting one or more chemical, biochemical or biological reactions in a disposable comprising one or more wells at one or more set point reaction temperatures, comprising: - at least one disposable comprising the one or more wells in a body, wherein the wells are capable of acting as vessel for the one or more chemical, biochemical or biological reactions requiring one or more prescribed reaction temperatures according to a prescribed protocol, said body comprising a flat bottom side building a first heating surface capable of homogeneously conducting heat into the wells and a flat upper side comprising well openings, optionally sealed by means of a thin sealing foil, building a second heating surface capable of homogeneously conducting heat in the wells; - one or more gradient tempering unit GTUx, each comprising at least one tempering block comprising at least two temperable Peltier elements contacting a thermoconductive block, opposite to the Peltier elements a flat-surfaced area for positioning the at least one disposable on the thermoconductive block, said tempering block being configured to generate a set linear temperature profile in the wells of the disposable(s) when contacting the thermoconductive block; - a control unit comprising one or more processors configured to control the gradient tempering unit(s) GTUx for the implementation of the prescribed protocol for the one or more chemical, biochemical, or biological reactions; wherein the gradient tempering block with set linear temperature profile comprises at least one Peltier element temperable at a first temperature T1 and at least one Peltier element temperable at a second temperature T2, wherein T1 is higher than T2 , and both Peltier elements are contacting the thermoconductive block outside of the positioning area for the disposable so that the linear temperature profile can be generated in the thermoconductive block between the two elements and conducted to the disposable while contacting the thermoconductive block. In particular, the solution can be used for cellular thermal shift assays (CETSA<®>), purified protein thermal shift assays (TSA) or any other protein denaturation or aggregation assays. The solution allows high throughput reactions or assays.

Anmelder

Firma

Bayer AG

Land

🇩🇪 Deutschland

Fachgebiete