Biomolekülanalyse mittels Erkennung von Fluoreszenzinterminitätssignalen

Details

Veröffentlichungs-Nr.

EP4379383

Aktenzeichen

EP22210671

Anmeldetag

30. November 2022

Veröffentlichung

5. Juni 2024

Rechtsraum

EP

IPC

G01N33/58G01N33/68G16B20/00

Abstract

The invention relates to a method for identifying a variant of a biomolecule among a plurality of variants of the biomolecule by these steps: A) Acquiring a fluorescence intermittence signal pattern for each variant. Each of the variants is labelled with a blinking fluorophore. B) Determining a fluorescence intermittency signal characteristic for each variant. C) Labelling at least one variant of the biomolecule in a sample with the fluorophore, and acquiring its fluorescence intermittency signal. D) Determining a presence of at least one fluorescence intermittency signal characteristic as determined in step B) in the acquired fluorescence intermittency signals from the sample. E) Establishing the presence, the quantity and/or localisation, of at least one variant of the biomolecule in the sample based on the fluorescence intermittency signal characteristic.

Anmelder

Firmen

Universität Zürich
Ecole Polytechnique Fédérale de Lausanne (EPFL)

Land

🇨🇭 Schweiz

Fachgebiete