Chimäre Proteine und Ihre Verwendung als Antigennachweissystem

Details

Veröffentlichungs-Nr.

EP4421084

Aktenzeichen

EP23382158

Anmeldetag

21. Februar 2023

Veröffentlichung

28. August 2024

Rechtsraum

EP

IPC

C07K14/245C07K14/24C12Q1/66C12Q1/02

Abstract

The present invention relates to a chimeric protein and a biosensor system based on said chimeric protein that enable the binding of an extracellular target antigen and the generation of a signal upon recognition. The chimeric protein of the present invention comprises (i) an antigen binding domain, (ii) at least one β-barrel domain from an autotransporter, and (iii) a reporter protein domain comprising a partner of a split protein luminescence system, wherein domain (ii) is bound to domain (i) by one end and to domain (iii) by the other end. The present invention also discloses a host cell comprising at least two chimeric proteins of the invention complementary each other as an in vitro antigen detection system.

Anmelder

Firma

Consejo Superior De Investigaciones Científicas

Land

🇪🇸 Spanien

Fachgebiete