Hochaktive Crispr-Basiseditoren durch Cas-Unterstützte Substratgebundene Gerichtete Evolution (caslid)

Details

Veröffentlichungs-Nr.

EP4458963

Aktenzeichen

EP23171650

Anmeldetag

4. Mai 2023

Veröffentlichung

6. November 2024

Rechtsraum

EP

IPC

C12N9/22C12N9/78C12N15/10C12N15/90

Abstract

The invention relates to a DNA modifying enzyme comprising or consisting of a DNA editing protein and a Cas protein and a peptide linker, wherein said DNA editing protein and said Cas protein are connected by said peptide linker; and wherein said DNA modifying enzyme has been obtained by directed molecular evolution of an original enzyme; and wherein said DNA modifying enzyme shows a base editing rate which is at least 10%, 20% or 30 %, preferably at least 40 %, more preferably at least 50 %, most preferably at least 60 % higher than the base editing rate of said wildtype enzyme. The invention relates further to a method of directed molecular evolution of a DNA modifying enzyme and to the use evolved DNA modifying enzymes in research, medicine and agriculture.

Anmelder

Firma

Technische Universität Dresden

Land

🇩🇪 Deutschland

Fachgebiete