Zellfreie Glycosylierung von Peptiden und Proteinen

Details

Veröffentlichungs-Nr.

EP4600371

Aktenzeichen

EP24156575

Anmeldetag

8. Februar 2024

Veröffentlichung

13. August 2025

Rechtsraum

EP

IPC

C12P21/00C07H11/04C07K2/00C12N9/10C12N9/12

Abstract

This invention concerns a covalent conjugate between a monosaccharide and/or glycan and the lipid carrier molecule phytol as well as a cell-free in vitro method and a cell free in vitro system for forming of a glycosylated peptide or polypeptide using said conjugate. A peptide or polypeptide comprising one or more glycosyl-moiety acceptor residues is contacted with a covalent conjugate between a monosaccharide and/or glycan and the lipid carrier molecule phytol in the presence of an oligosaccharyltransferase to form a glycosylated peptide or polypeptide. The invention also concerns a peptide or polypeptide with a glycosyl-moiety acceptor residue having an exogenous N-linked glycosylation sequence L(A/V)NYT, as well as an N-glycosylated conjugate of said peptide or polypeptide.

Anmelder

Firma

ETH Zurich

Land

🇨🇭 Schweiz

Fachgebiete