Probenvorbereitungsverfahren und Vorrichtung zur Herstellung einer Verglasten Probenschicht auf einem Probenträgergitter für die Anschliessende Kryoelektronenmikroskopie
Anmelder: European Molecular Biology Laboratory 🇩🇪
Details
- Veröffentlichungs-Nr.
- EP4711738
- Anmeldetag
- 17. September 2024
- Veröffentlichung
- 18. März 2026
- Rechtsraum
- EP
- IPC
- G01N1/42(MCQUEEN TREVOR ALLEN et al.)
Abstract
A sample preparation method and device for preparing a vitrified sample layer (1) on a sample holder grid (3) for subsequent analysis of physical sample characteristics, particularly for subsequent cryo electron microscopy, are presented. The method comprises:providing the sample holder grid (3) with sample solution (5) on at least one surface of the sample holder grid (3);- spreading the dispensed sample solution (5) along the surface (7) of the sample holder grid (3) using one or a sequence of multiple shock pressure waves (9) being generated by a shock pressure wave generator (11) emitting and directing the one or multiple shock pressure waves (9) via at least one nozzle (13) onto the surface (7) of the sample holder grid (3);- vitrifying the spread sample solution (5) by exposing it to a cooling liquid (17) at cryogenic temperatures.
Anmelder
- Firma
- European Molecular Biology Laboratory
- Land
- 🇩🇪 Deutschland