Hochdurchsatzherstellung von Geschichteten Co-Kulturen

Details

Veröffentlichungs-Nr.

EP4715041

Anmeldetag

20. September 2024

Veröffentlichung

25. März 2026

Rechtsraum

EP

IPC

C12N5/071(BAROUD CHARLES et al.)(KUMACHEV EUGENIA et al.)

Abstract

The present invention concerns a method for preparing a spherical core-shell skin model, said method comprising the steps of: a) forming microdroplets of an aqueous solution comprising fibroblasts and collagen, in an oil, wherein the formed collagen microdroplets comprise at least one fibroblast per microdroplet; b) gelling at least a portion of the formed collagen microdroplets comprising said at least one fibroblast, thereby obtaining collagen beads comprising at least one fibroblast per bead; c) replacing the oil surrounding the collagen beads comprising said at least one fibroblast with a culture medium, d) contacting (i) the collagen beads comprising said at least one fibroblast with (ii) keratinocytes, and e) incubating (i) said collagen beads with (ii) said keratinocytes for a period of time enabling the formation of an external layer by said keratinocytes around said collagen beads, thereby obtaining a spherical core-shell skin model, the core comprising at least one fibroblast and the shell comprising at least one keratinocyte.

Anmelder

Firmen

L V M H RECHERCHE
Ecole Polytechnique
Centre National de la Recherche Scientifique (CNRS)
Institut Pasteur

Land

🇫🇷 Frankreich