Einstufiges Verfahren zur Ausfällungs- und Farbentwicklungsdetektion einer Nitrocellulose (nc)-Membran für Mikro-/nanokunststoffe

Details

Veröffentlichungs-Nr.

EP4729941

Anmeldetag

28. November 2024

Veröffentlichung

22. April 2026

Rechtsraum

EP

Abstract

A one-step precipitation and color development detection method of a nitrocellulose (NC) membrane for micro/nano plastics was provided, including the following steps: S1, preparing a micro/nano plastic-ovalbumin (OVA) conjugate; S2, preparing an NC membrane coated with the micro/nano plastic-OVA conjugate; S3, putting the NC membrane coated with the micro/nano plastic-OVA conjugate into a polystyrene antibody solution and a sample solution and incubating for 1 h at 37°C, and then rinsing the NC membrane; S4, diluting goat anti-mouse IgG-horseradish peroxidase (HRP) by 1w fold and then putting in a centrifuge tube, putting the NC membrane into the centrifuge tube, and then incubating for 30 min at 37°C; and S5, washing the NC membrane 4-5 times, completely immersing the NC membrane into a precipitating tetramethylbenzidine (TMB) for 30 s and then taking out the NC membrane at room temperature for development, followed by detecting the concentration of micro/nano plastics.

Anmelder

Firma

Wuhan Textile University

Land

🇨🇳 China