Tracer-Verbindungen zur Ganzzellmarkierung
Anmelder: Charité - Universitätsmedizin Berlin Körperschaft des öffentlichen Rechts 🇩🇪
Details
- Veröffentlichungs-Nr.
- EP4733763
- Anmeldetag
- 25. Oktober 2024
- Veröffentlichung
- 29. April 2026
- Rechtsraum
- EP
Abstract
The invention relates to a tracer compound for whole-cell labelling, comprising: a non-streptavidin-binding biotin analogue, at least one connecting moiety comprising a functional group configured for a click-chemistry reaction, and at least one cross-linkable moiety, preferably wherein the cross-linkable moiety is a (para)formaldehyde cross-linkable group, and the non-streptavidin-binding biotin analogue enables the tracer compound to diffuse within a biological cell with essentially the same properties as biocytin and/or neurobiotin, preferably enabling the detection of cellular protrusions, such as dendrites, dendritic spines and axons. The invention further relates to methods for constructing such tracer compound and for labelling a cell therewith. The invention further relates to kits for carrying out the method according to the invention.
Anmelder
- Firma
- Charité - Universitätsmedizin Berlin Körperschaft des öffentlichen Rechts
- Land
- 🇩🇪 Deutschland