Verfahren zum Nachweis einer Zielsubstanz und Reagenz zur Untersuchung einer Zielsubstanz

Details

Veröffentlichungs-Nr.

EP4737586

Anmeldetag

3. November 2025

Veröffentlichung

6. Mai 2026

Rechtsraum

EP

IPC

(KAKEGAWA NORISHIGE et al.)(CANON KK et al.)

Abstract

Provided are a highly sensitive detection method for a target substance and a reagent for inspection thereof. Specifically, provided is a method of detecting a target substance that is an enzyme reaction-related substance, the method including: a first step of mixing a specimen and a reagent to obtain a liquid sample containing hydrogen peroxide, a peroxidase (40), a phenol derivative, a luminescent particle (10) and hydrophilic polymers (30) each having a binding functional group; a second step of generating an aggregate (50) of the hydrophilic polymers (30) including the luminescent particle (10) through the binding functional groups of the hydrophilic polymers (30) based on a reaction of the hydrogen peroxide, the peroxidase (40) and the phenol derivative, which occurs when the target substance is present in the liquid sample; and a third step of obtaining a value related to fluorescence anisotropy of the liquid sample in the second step.

Anmelder

Firma

Canon Kabushiki Kaisha

Land

🇯🇵 Japan