Zellablösungsverfahren und Zellablösungsvorrichtung

Details

Veröffentlichungs-Nr.

EP4741489

Anmeldetag

16. Juli 2024

Veröffentlichung

23. Januar 2025

Rechtsraum

EP

IPC

C12M3/00, C12N5/071

Abstract

Provided are a cell detachment method and a cell detachment device that enable vibration design suitable for target cells and detachment of adherent cells at a high detachment rate. The cell detachment method of detaching cells adhering to a culture surface of a culture substrate from the culture surface includes a detachment step of detaching the cells from the culture surface by vibrating a vibrating body under a state in which a first acoustic transmission medium and a second acoustic transmission medium are arranged between the culture substrate and the vibrating body, and under a state in which at least one of the following (a) or (b) is satisfied: (a) the culture substrate is in contact with the first acoustic transmission medium and the second acoustic transmission medium; or (b) the vibrating body is in contact with the first acoustic transmission medium and the second acoustic transmission medium. In the cell detachment method, the first acoustic transmission medium and the second acoustic transmission medium are different from each other, the first acoustic transmission medium being a solid at room temperature, the second acoustic transmission medium being a liquid or a solid with flowability at room temperature.

Anmelder

Firma

Canon Kabushiki Kaisha

Land

🇯🇵 Japan