Massenspektrometrieverfahren für Datenabhängigen Quadrupolbetrieb

Details

Veröffentlichungs-Nr.

EP4749680

Anmeldetag

21. November 2024

Veröffentlichung

27. Mai 2026

Rechtsraum

EP

IPC

H01J49/00

Abstract

The invention relates to a method for analyzing a sample comprising one or more molecules of interest by a mass spectrometer, comprising ionizing a multitude of molecules and/or fragments thereof comprised within a sample to obtain a multitude of molecule ions (source ions), and performing mass spectrometry (MS) analysis comprising a. analyzing the molecule ions and/or fragments thereof in a MS1 measurement, thereby acquiring ion mass spectral data of at least a fraction of the molecule ions and/or fragments thereof (source ions) comprising their mass to charge ratio (m/z), b./c. isolating a first fraction and a second of the molecule ions (precursor ions) having a m/z within a first m/z range (mz1; isolation window) and a second m/z range (mz2; isolation window), using a mass filter device, wherein the first m/z range is selected based on the ion mass spectral data acquired in step a. or one or more iterations of step a. and/or e., and wherein the second m/z range (mz2) overlaps with the first m/z range (mz1), d. fragmenting at least a fraction of the molecule ions (precursor ions) isolated in steps b. and c., separately, to obtain a multitude of biomolecule fragment ions from each of the respective fragmented molecule ions (precursor ions), and e. analyzing the multitude of biomolecule fragment ions in one or more MS2 measurement, thereby acquiring ion mass spectral data of the analyzed biomolecule fragment ions.

Anmelder

Firma

Charité - Universitätsmedizin Berlin Körperschaft des öffentlichen Rechts

Land

🇩🇪 Deutschland

Fachgebiete