Identifizierung von Verwandten Paaren von Liganden und Rezeptoren

Details

Veröffentlichungs-Nr.

EP4752221

Anmeldetag

19. Dezember 2019

Veröffentlichung

3. Juni 2026

Rechtsraum

EP

IPC

C12Q1/6806, C12N15/10C12N15/10

Abstract

The invention concerns a method for identifying cognate pairs of a ligand species and a receptor species, comprising co-compartmentalising ligand species and receptor species to form a set of microreactors, each microreactor comprising at least one ligand species and preferably at least one receptor species; assaying the recognition between ligands and receptors in each microreactor and based on this assay, classifying each microreactor as positive when at least one ligand species and at least one receptor species in the microreactor recognize one with the other or negative when no ligand species and no receptor species recognize in the microreactor; identifying ligand species and receptor species contained in each positive microreactor; establishing a subset of positive microreactors containing the same receptor species; determining the probability that in a given subset of positive microreactors containing the same receptor species, the ligand species recognizing the receptor species corresponds to the most frequent co-compartmentalized ligand species; and if the determined probability is greater than a predetermined threshold, identifying as a cognate pair the receptor species and the most frequent co-compartmentalized ligand species.

Anmelder

Firmen

Paris Sciences et Lettres
Centre National de la Recherche Scientifique
Institut Curie
The President and Fellows of Harvard College
HiFiBiO (HK) Limited
Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris
Institut National de la Santé et de la Recherche Médicale

Land

🇫🇷 Frankreich