Verfahren zur Herstellung eines Oligonukleotids

Details

Veröffentlichungs-Nr.

EP4755897

Anmeldetag

1. August 2024

Veröffentlichung

6. Februar 2025

Rechtsraum

EP

Abstract

The present application provides a method for producing an oligonucleotide, the method including: cleaving an oligonucleotide from a conjugate containing the oligonucleotide, a cleavable linker, and a support in a solution containing an alkylamine and an organic solvent; and optionally deprotecting a protecting group of the oligonucleotide, wherein the cleavable linker has a cyclic vicinal diol structure; and the organic solvent is selected from an ether, a cyclic amide, and a cyclic urea. According to the present production method, an oligonucleotide can be cleaved with high purity and yield from a conjugate containing the oligonucleotide, a cleavable linker, and a support by suppressing the generation of cleavable-linker-attached by-products and degradation products.

Anmelder

Firma

Nippon Shokubai Co., Ltd.

Land

🇯🇵 Japan