Verfahren und Vorrichtung zur Vorbereitung von Proben Fuer die Kryoelektronenmikroskopie

Details

Veröffentlichungs-Nr.

EP4756400

Anmeldetag

3. Dezember 2024

Veröffentlichung

10. Juni 2026

Rechtsraum

EP

IPC

G01N1/28, G01N1/36, G01N1/42

Abstract

The invention relates to an Apparatus and a Method for preparing samples for electron cryomicroscopy, the method comprising the steps of:• Placing (100) a specimen holder (H) within a vacuum chamber (C),• Generating (200) a vacuum inside the vacuum chamber (C),• Cooling (300) the specimen holder (H) to a temperature such that an ejected sample could vitrify on the specimen holder (H),• Using the nozzle (N) to eject (400) a sample towards the specimen holder (H), whereby while ejecting the specimen holder (H) and / or the nozzle (N) is /are moved relative to each other such that the resulting relative movement of the specimen holder (H) with respect to the nozzle (N) changes the distance between the nozzle (N) and the specimen holder (H) as well as providing for a movement in a plane normal to the distance of the nozzle (N) relative to the specimen holder (H), such that the impacting points of the ejected sample lead to non-overlapping impact points of vitrified samples on the specimen holder (H).

Anmelder

Firmen

Universität Hamburg KöR
Deutsches Elektronen-Synchrotron DESY
Leibniz-Institut für Virologie

Land

🇩🇪 Deutschland