Verfahren und Kits zur Isolierung einer Zielnukleinsäure durch In-Gel-Affinitätselektrophorese

Details

Veröffentlichungs-Nr.

EP4763992

Anmeldetag

23. Dezember 2024

Veröffentlichung

24. Juni 2026

Rechtsraum

EP

IPC

C12Q1/6806

Abstract

1. The present invention relates to methods and kits for isolating a target nucleic acid by in-gel affinity electrophoresis. The method comprises the steps of: a. covalently immobilizing an anchor oligonucleotide during polyacrylamide polymerization in a polyacrylamide binding gel, wherein the anchor oligonucleotide comprises (a) a binding element that covalently binds to acrylamide of the polyacrylamide binding gel and (b) a first coupling nucleotide sequence that is complementary to a nucleotide sequence of a bridge oligonucleotide to hybridize the anchor oligonucleotide to the bridge oligonucleotide, wherein the bridge oligonucleotide comprises (a) a second coupling nucleotide sequence that is complementary to the first coupling sequence of the anchor oligonucleotide and (b) a landing site with at least one complementary target nucleotide sequence, b. hybridization of the anchor oligonucleotide to the bridge oligonucleotide, c. performing a polyacrylamide gel electrophoresis (PAGE) to enrich target nucleic acid by in-gel hybridization, d. depletion of undesired species by in-gel nucleic acid depletion, e. isolating the hybridized target nucleic acid from the binding gel.

Anmelder

Firma

Johannes Gutenberg-Universität Mainz

Land

🇩🇪 Deutschland

Fachgebiete