Verfahren zum Screening auf Peptide unter Verwendung mehrerer Bibliotheken

Details

Veröffentlichungs-Nr.

EP4768598

Anmeldetag

10. Oktober 2024

Veröffentlichung

17. April 2025

Rechtsraum

EP

IPC

C12Q1/6844, C40B40/02

Abstract

The present invention provides a method for screening for a candidate peptide capable of binding to a target molecule, the method including the steps of: (1) preparing a plurality of nucleic acid display libraries containing a barcoded peptide-nucleic acid complex, wherein the barcoded peptide-nucleic acid complex contains a nucleic acid moiety and a peptide moiety, the nucleic acid moiety contains a barcode sequence and a nucleic acid sequence encoding the peptide, and the plurality of nucleic acid display libraries are nucleic acid display libraries, each of which is independently produced by translation using a cell-free translation system; (2) mixing the plurality of nucleic acid display libraries to prepare a mixed nucleic acid display library; (3) bringing the mixed nucleic acid display library into contact with the target molecule; and (4) amplifying a nucleic acid corresponding to the nucleic acid moiety of the barcoded peptide-nucleic acid complex bound to the target molecule, using a barcode primer.

Anmelder

Firma

Chugai Seiyaku Kabushiki Kaisha

Land

🇯🇵 Japan

Fachgebiete