Virusherstellungsverfahren und Verfahren zur Einführung von Nukleinsäure in eine Zelle
Anmelder: FUJIFILM Corporation 🇯🇵
Details
- Veröffentlichungs-Nr.
- EP4772616
- Anmeldetag
- 28. August 2024
- Veröffentlichung
- 6. März 2025
- Rechtsraum
- EP
Abstract
An object of the present invention is to provide a method for improving a proportion of capsids containing a gene of interest GOI in a method for producing a virus, and to provide a method for improving a proportion of capsids containing a gene of interest GOI in a method for introducing a nucleic acid into cells, the method including a nucleic acid introduction step of adding a nucleic acid to a suspension containing cells. According to the present invention, there is provided a method for producing a virus, the method including a nucleic acid introduction step of adding a nucleic acid to a suspension containing cells having a density of 10 × 10<sup>6</sup> cells/mL or more, and a culture step of culturing the cells into which the nucleic acid has been introduced, in which the nucleic acid is added in a case where a population cell doubling time of the suspension satisfies 35.4 hours or less.
Anmelder
- Firma
- FUJIFILM Corporation
- Land
- 🇯🇵 Japan
Japanischer Konzern, hervorgegangen aus der Fotofilmherstellung, heute aktiv in Bildgebung, Dokumentenlösungen, Materialwissenschaften sowie Diagnostik und Arzneimittelherstellung.
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Vertreten von
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Hoffmann Eitle
Hoffmann Eitle · München