Genomeditiertechnik

Details

Veröffentlichungs-Nr.

EP4772626

Anmeldetag

29. August 2024

Veröffentlichung

6. März 2025

Rechtsraum

EP

IPC

C12N15/09, C12N15/63

Abstract

Provided is a method for deleting, substituting or inserting a target nucleotide sequence in a genome editing target region in an organism or a cell to be subjected to genome editing. The method comprises: (1) a step for introducing a type II restriction endonuclease recognition sequence into the genome editing target region; and (2) a step for expressing a type II restriction endonuclease capable of recognizing the introduced type II restriction endonuclease recognition sequence in the organism or the cell to cause double strand break (DSB) in the genome. In the method, after performing the steps (1) and (2), the target region is edited through homologous recombination repair with the genome editing gene sequence in the organism or the cell. In the organism or the cell, the GC content in the genome is 65% or more. As a result, it becomes possible to provide a genome editing technique for all organisms, particularly actinomycetes, wherein an organism or a cell obtained after the modification does not correspond to a recombinant and an off-target effect can be avoided.

Anmelder

Firmen

The School Corporation Kansai University
JNC Corporation

Land

🇯🇵 Japan