Reaktor zur Erzeugung von Konzentrationsgradienten von Fluiden

Details

Veröffentlichungs-Nr.

EP4796626

Anmeldetag

24. Februar 2025

Veröffentlichung

26. August 2026

Rechtsraum

EP

Abstract

The invention relates to a gradient reactor for generating a concentration gradient, comprising: a) a first layer comprising i) at least one fluid inlet for an injection of at least one fluid, ii) at least one dilutant inlet for an injection of a dilutant, and iii) at least one outlet for an ejection of the at least one fluid, b) a second layer, c) a third layer suitable for culturing microorganisms or cells, wherein a concentration gradient is formable in the second layer, and the concentration gradient is transferable from the second layer to the third layer. The invention further relates to a system comprising i) the gradient reactor according to any one of the preceding claims, wherein the gradient reactor optionally further comprises pumps, preferably micropumps, for automated injection of the at least one fluid and the dilutant, ii) a camera system, and iii) a computer program comprising instructions, optionally wherein the instructions, when the program is executed by a computer, cause the computer to analyze the growth, proliferation and/or survival of the microorganisms or cells. The invention also relates to the use of the gradient reactor according to any one of the preceding claims, for the generation of overlapping stationary or dynamic concentration gradients, for analyzing the efficacy or effect of different biologically active compounds, for assessing possible combinatory therapies, for assessing the probability of future resistance to the different biologically active compounds, or for analyzing pharmacokinetics. The invention also relates to a method for generating one or more concentration gradients, comprising: i) providing a gradient reactor according to the invention, or a system according to the invention, ii) simultaneously injecting the dilutant and the at least one fluid, into the at least one dilutant inlet and the at least one fluid inlet, respectively, iii) generating one or more concentration gradients in the second layer, iv) wherein the one or more concentration gradients transfer from the second layer to the third layer, wherein the third layer is suitable for culturing microorganisms or cells.

Anmelder

Firmen

Charité - Universitätsmedizin Berlin Körperschaft des öffentlichen Rechts
Freie Universität Berlin, Körperschaft des öffentlichen Rechts

Land

🇩🇪 Deutschland