Verfahren zur Transduktion von Natürlichen Killerzellen (nk) mit Retroviralen Vektoren

Details

Veröffentlichungs-Nr.

EP4803613

Anmeldetag

26. Februar 2026

Veröffentlichung

9. September 2026

Rechtsraum

EP

Abstract

The present invention provides an in-vitro method for the generation of a composition comprising genetically modified NK cells, the method comprising the steps a) enrichment of NK cells from a sample comprising NK cells and other cells, b) genetic modification of said enriched NK cells by transduction with a retroviral vector, c) cultivation of said genetically modified NK cells, wherein said genetic modification step b) is performed in the presence of a tyrosine kinase (TK) inhibitor, wherein the step of genetic modification of said enriched NK cells comprise the addition of said TK inhibitor to said enriched NK cells not later than 24 hours after the contact of the retroviral vector with the enriched NK cells, and wherein said TK inhibitor is added up to 24 hours before said genetic modification step to said enriched NK cells.

Anmelder

Firma

Miltenyi Biotec B.V. & Co. KG

Land

🇩🇪 Deutschland