Optisches System für einen Fluoreszenzbildgeber

Details

Veröffentlichungs-Nr.

EP4803886

Anmeldetag

4. März 2025

Veröffentlichung

9. September 2026

Rechtsraum

EP

IPC

G01N21/64

Abstract

The present invention relates to optical imaging systems, specifically fluorescence imaging systems. An optical system for a fluorescence imager is disclosed, comprising a laser source (1) configured to generate a collimated excitation laser beam, a beam splitter (2) configured to receive the excitation laser beam and divide it into two substantially equal beams, and a receiving structure (3'') configured to accommodate a target vessel (3) configured to hold a target containing fluorophores. The system includes two substantially identical excitation systems (4', 4'') positioned symmetrically around the target vessel (3) on opposite lateral sides, ensuring uniform beam energy distribution across the target. Each excitation system (4', 4'') comprises a fiber optic cable (41) configured to transmit a focused beam, a first half-ball lens (42) configured to focus the beam into the fiber optic cable (41), a second half-ball lens (43) configured to recollimate the beam upon exiting the fiber optic cable (41), and a mirror (45) positioned at an angle (X) to direct the recollimated beam towards the target vessel (3), reflecting the beam at a total angle of approximately twice the mirror angle (2X). An imaging system (5) is configured to capture fluorescence emission from the target vessel (3) and includes an aperture (51), one or more optical filters (52', 52'') configured to attenuate excitation light, an imaging lens (53), and a photosensor (54) configured to capture fluorescence light emitted from the target. The optical filters (52', 52'') are positioned on opposite sides of the imaging lens (53), ensuring selective transmission of fluorescence emissions while blocking undesired excitation light. The system provides precise optical alignment, uniform energy distribution, and efficient fluorescence detection, enhancing imaging performance and signal clarity.

Anmelder

Firma

Bioscience Media, SIA

Land

🇱🇻 Lettland

Fachgebiete