Verfahren zur Herstellung von Dna/rna-Oligo- und Polynukleotiden

Details

Veröffentlichungs-Nr.

EP4821938

Anmeldetag

10. April 2025

Veröffentlichung

14. Oktober 2026

Rechtsraum

EP

IPC

C07H1/00, B01J19/00, C07H21/00

Abstract

The present invention is directed to a method for the continuous flow preparation of oligonucleotide compounds using solid-phase flow chemistry implemented in two separated process chambers in continuous flow communication, wherein the first process chamber is an activation chamber (upstream chamber) for activating a nucleotide compound, and the second process chamber is a coupling chamber (downstream chamber) for coupling the activated nucleotide compound from the activation chamber to a solid-phase support (SPS)-bound mono-, di-, tri-, oligo- or polynucleotide compound, wherein both process chambers comprise a fluid-permeable divider for separating the activation chamber (a) from the coupling chamber (b) and its solid-phase bound mono-, di-, tri-, oligo- or polynucleotides. The present invention further relates to an alternative method for the continuous flow preparation of oligonucleotide compounds using solid-phase flow chemistry, wherein the 5'-hydroxy of a mono-, di-, tri-, oligo- or polynucleotide bound to a solid phase support (SPS) is activated and reacted with the 3'-hydroxy of a nucleotide to prepare a di-, tri, oligo- or polynucleotide compound in continuous flow preparation.

Anmelder

Firmen

Universität Zürich
Novartis AG

Land

🇨🇭 Schweiz

Fachgebiete