Plasmidvektor, Verfahren zum Erkennen der Genpromoteraktivität und Assaykit

Details

Veröffentlichungs-Nr.

EP2562258

Aktenzeichen

EP12181691

Anmeldetag

24. August 2012

Veröffentlichung

1. Juli 2015

Erteilung

1. Juli 2015

Rechtsraum

EP

IPC

C12N15/69C12N15/85

Abstract

According to one embodiment, a plasmid vector (1) includes a gene promoter (10), a first gene (21), a second gene (25), an internal ribosome entry site (23), a transcription termination signal sequence (27) and a replication origin sequence (30). The first gene (21) encodes a reporter protein for visualizing the activity of the gene promoter (10) which is disposed at the downstream of the gene promoter (10). The second gene (25) is disposed at the downstream of the gene promoter (10) and encodes a replication origin-binding protein. The internal ribosome entry site (25) is disposed between the first gene (21) and the second gene (25). The transcription termination signal sequence (27) encodes a signal for terminating the transcription of the first gene (21) and the second gene (25). The replication origin sequence (27) is recognized by the replication origin-binding protein.

Anmelder

Firmen

Kabushiki Kaisha Toshiba
Toshiba Medical Systems Corporation

Land

🇯🇵 Japan

Fachgebiete