Verfahren zur Herstellung von duplizierter Translokation von optionalen Bereichen in Aspergillus-Chromosomen

Details

Veröffentlichungs-Nr.

EP2647714

Aktenzeichen

EP13162003

Anmeldetag

2. April 2013

Veröffentlichung

1. März 2017

Erteilung

1. März 2017

Rechtsraum

EP

IPC

C12N15/80

Abstract

The purpose of the present invention is to provide a technique to duplicate and translocate any large region in a chromosome of a fungus belonging to Aspergillus across a wide range, so that it will possible to stably and systematically acquire an Aspergillus strain having a novel trait, which was un-acquirable by conventional techniques. The present invention relates to a transformant of the fungus belonging to Aspergillus wherein a transformation marker gene with deficiency of a terminal part at the 5' or 3' end of its coding region is integrated into an outside of a target region in a chromosome of the fungus subject to duplicated translocation, and a transformation marker gene with deficiency of a terminal part at the 3' or 5' end of its coding region is integrated into an outside of a region in another chromosome of the fungus to be replaced with the target region.

Anmelder

Firma

Kikkoman Corporation

Land

🇯🇵 Japan

Fachgebiete