Vorrichtung und Verfahren zur Ausführung einer digitalen PCR
Anmelder: IMEC VZW 🇧🇪
Details
- Veröffentlichungs-Nr.
- EP2878374
- Aktenzeichen
- EP13195075
- Anmeldetag
- 29. November 2013
- Veröffentlichung
- 12. Mai 2021
- Erteilung
- 12. Mai 2021
- Rechtsraum
- EP
- IPC
- B01L3/00B01L7/00
Abstract
A micro-fluidic device 100 for performing digital PCR is presented. The device comprises: a semiconductor substrate; a first micro-fluidic channel 104, comprising an inlet 102 and an outlet 103, embedded in the semiconductor substrate; a heating element 101 thermally coupled to the first micro-fluidic channel 104; a droplet generator 107 connected to the inlet 102 of the first micro-fluidic channel 104 for generating droplets and pumping generated droplets at a flow rate into the first micro-fluidic channel 104; characterized in that: the heating element 101 is a single heating element connected to a temperature control unit 111 configured to cycle the temperature of the complete first micro-fluidic channel 104 through at least two temperature values; and wherein the flow rate of the droplet generator 107 is adaptable. Further, a method to perform digital PCR is presented using the micro-fluidic device 100.
Anmelder
- Firma
- IMEC VZW
- Land
- 🇧🇪 Belgien
Fachgebiete
-
Patent Department IMEC
Patent Department IMEC · Leuven