Verfahren zur Lipidanalyse mittels Massenspektrometrie und Massenspektrometer

Details

Veröffentlichungs-Nr.

EP3422388

Aktenzeichen

EP18179957

Anmeldetag

26. Juni 2018

Veröffentlichung

5. April 2023

Erteilung

5. April 2023

Rechtsraum

EP

IPC

H01J49/00G01N33/92

Abstract

Lipid-derived ions captured within an ion trap are irradiated with hydrogen radicals to induce the reaction of hydrogen extraction (S1, S2). A precursor-ion isolation process is subsequently performed (S3), and the precursor ion is dissociated by low-energy collision-induced dissociation (S4). The thereby generated product ions are subjected to mass spectrometry to create a product-ion spectrum (S5, S6). Since the dissociation achieved by such a procedure does not cause hydrogen rearrangement, a peak pair having a mass difference of +12 Da characteristic of the unsaturated bond site certainly appears on the product-ion spectrum. By searching for this peak pair, the unsaturated bond site can be located (S7, S8). By such a method, a lipid-structure analysis including the determination of the position of the unsaturated bond site in a lipid can be performed in a stable and accurate manner without requiring derivatization or other cumbersome pretreatments.

Anmelder

Firma

Shimadzu Corporation

Land

🇯🇵 Japan

Fachgebiete