Vorrichtung und Verfahren zur Grössenselektiven Partikelabscheidung, Einfangen und Manipulation von Mikro- und Nanopartikeln zur Molekularen Detektion
Anmelder: PAUL SCHERRER INSTITUT 🇨🇭
Details
- Veröffentlichungs-Nr.
- EP3778024
- Aktenzeichen
- EP19192141
- Anmeldetag
- 16. August 2019
- Veröffentlichung
- 3. August 2022
- Erteilung
- 3. August 2022
- Rechtsraum
- EP
- IPC
- B01L3/00G01N33/543// G01N21/552G01N21/65
Abstract
It is the objective of the present invention to enable molecular detection at very small target molecule concentrations (a few pg/ml) in presence of whole blood, urine etc., a robust method is required which can provide conclusive information of multiple biomarkers on a single chip. This objective is achieved according to the present invention by a lab on a chip (LoC) device (2) for size-selective particle separation, trapping, and manipulation of micro/nanoparticles from a flow of a sample solution for molecular detection, comprising: a) an in-port area (4) for the reception of a sample solution; b) a microparticle separation area (6) and a macroparticle separation area (8); c) a nanoparticle sorting and separation area (10); d) a functionalized nanoparticle trapping and detection area (12); and e) an out-port area (20) for the waste sample solution. Further, the objective mentioned above is achieved according to the present invention by a method for size-selective particle separation, trapping and a manipulation of micro/nanoparticles from a flow of a sample solution for molecular detection; comprising the steps of: a) providing a sample solution to be investigated; b) conveying the sample solution through a LoC device according to any of the preceding claims 1 to 7. Therefore, the present device represents a lab-on-a-chip platform that allows for particle separation, sorting and trapping at specific locations in the device for molecular sensing in the range of nano- to picomolar concentration. In combination with optical detection using surface-enhanced Raman spectroscopy (SERS) and localized surface plasmon resonance (LSPR), a highly sensitive (∼10 pg/ml) molecular detection can be achieved.
Anmelder
- Firma
- PAUL SCHERRER INSTITUT
- Land
- 🇨🇭 Schweiz
Das Paul Scherrer Institut ist das größte Forschungszentrum für Natur- und Ingenieurwissenschaften der Schweiz mit Sitz in Villigen und Anbindung an Großforschungsanlagen wie Synchrotron und Freie-Elektronen-Laser. Die Patente betreffen vor allem Mess- und Prüftechnik sowie Halbleiterelemente, ergänzt um Medizintechnik, Verfahrenstechnik und Optik. Anmeldungen erfolgen kontinuierlich seit 2000.
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