Laserabtastmikroskop und Verfahren zur Bestimmung einer Position eines Fluorophors

Details

Veröffentlichungs-Nr.

EP3828614

Aktenzeichen

EP19215601

Anmeldetag

12. Dezember 2019

Veröffentlichung

16. August 2023

Erteilung

16. August 2023

Rechtsraum

EP

IPC

G02B21/00

Abstract

A laser scanning microscope comprising a light source configured to form an illumination light beam, said illumination light beam having a transverse light intensity profile comprising an intensity minimum, a scanning element configured to scan a target area of a specimen with the illumination light beam along a closed trajectory, a detector configured to detect fluorescence light emitted by a fluorophore within the target area of the specimen, said fluorophore being excited by the illumination light beam, and a processor configured to determine an intensity distribution of the fluorescence light as a function of time and to determine a position of the fluorophore within the target area based on the intensity distribution of the fluorescence light.

Anmelder

Firma

Leica Microsystems CMS GmbH

Land

🇩🇪 Deutschland

Fachgebiete