Schrägflächenmikroskop zur Bildgebung einer Probe

Details

Veröffentlichungs-Nr.

EP3893039

Aktenzeichen

EP20169056

Anmeldetag

9. April 2020

Veröffentlichung

23. August 2023

Erteilung

23. August 2023

Rechtsraum

EP

IPC

G02B21/00G02B21/36G02B27/28

Abstract

An oblique plane microscope (100, 400, 500, 600, 700) for imaging a sample (104) comprises a single objective (102) configured to illuminate a plane (106, 606) of the sample (104) with a light sheet and to collect detection light (110, 610) from the illuminated plane (106, 606); a beam splitting system (112, 512, 712) configured to split the collected detection light (110, 610) into first and second two detection light bundles (114, 116) propagating along first and second optical paths (OP1, OP2), respectively; a first intermediate imaging system (126) configured to generate a first intermediate image of the illuminated plane (106, 606) from the first detection light bundle (114) in a first intermediate image space (130), said first intermediate imaging system (126) comprising a first objective (132) and a first reflecting element (134) which is positioned in the first intermediate image space (130) and configured to reflect the first detection light bundle (114) back into the first objective (132); a second intermediate imaging system (128) configured to generate a second intermediate image of the illuminated plane (106, 606) from the second detection light bundle (116) in a second intermediate image space (140), said second intermediate imaging system (128) comprising a second objective (142) and a second reflecting element (144) which is positioned in the second intermediate image space (140) and configured to reflect the second detection light bundle (116) back into the second objective (142); and a detection system (146, 546, 746) configured to detect the first and second detection light bundles (114, 116) reflected back into the first and second objectives (132, 142), respectively, for imaging the sample (104).

Anmelder

Firma

Leica Microsystems CMS GmbH

Land

🇩🇪 Deutschland

Fachgebiete