Verfahren und Vorrichtung zum Nachweis einer Konzentration von mindestens einem Analyten in einer Probe
Anmelder: Technische Universität München 🇩🇪
Details
- Veröffentlichungs-Nr.
- EP4592678
- Aktenzeichen
- EP24154199
- Anmeldetag
- 26. Januar 2024
- Veröffentlichung
- 30. Juli 2025
- Rechtsraum
- EP
- IPC
- G01N33/543B01L3/00G01N27/74
Abstract
The invention relates to a method for detecting a concentration of an analyte (10) in a sample (20), the method comprising: mixing capture elements (30), preferably capture elements comprising beads, more preferably comprising magnetic beads, into the sample (20), the capture elements(30) having capture binder elements (35,36,37); binding the analyte (10) to at least one capture element (30) via a capture binder element (35, 36, 37) such that at least one analyte bonded capture element (30) is formed; flowing the sample (20) through a channel (40) having a substrate (42), the substrate (42) having surface binder elements (44, 45, 46); moving the capture elements (30) to the substrate (42) with an external force (6o), preferably with a magnetic force; rolling the capture elements (30) along/on the substrate (42) and changing the velocity of said at least one analyte bonded capture element (30) by a binding interaction between a surface binder element (44, 45, 46) of the substrate (42) and the analyte (10) bound by the respective capture binder element (35, 36, 37); determining a concentration of the analyte (10) using a sensor unit (70) by determining a change of velocity of the capture elements (30) along the channel and/or by determining a depletion of the capture elements (30) along the channel (40), and/or determining binding kinetics, preferably binding constants, between the analyte (10) and the capture binder elements (35, 36, 37) and/or between the analyte (10) and the surface binder elements (44, 45, 46), wherein the binding kinetics are determined using the sensor unit (70) by determining the change of velocity of the capture elements (30) along the channel (40). The invention further relates to a device (100).
Anmelder
- Firma
- Technische Universität München
- Land
- 🇩🇪 Deutschland
Deutsche technische Universität mit Sitz in München, betreibt Forschung und Lehre in Ingenieur-, Natur- und Lebenswissenschaften sowie Medizin.
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Boehmert & Boehmert geht auf die Zulassung von Dr. Karl Boehmert 1931 in Berlin zurück. Mit Standorten in Deutschland, Alicante, Paris und Shanghai bietet die Kanzlei IP aus einer Hand und legt besonderen Wert auf persönliche Mandantenbetreuung statt Anonymität.