Reinigung Hybridisierter Rna-Polynukleotide

Details

Veröffentlichungs-Nr.

EP4722382

Anmeldetag

9. Dezember 2024

Veröffentlichung

8. April 2026

Rechtsraum

EP

IPC

(GOLDRICK MARIANNA et al.)(MBIOMICS GMBH et al.)

Abstract

A method of purifying hybridized RNA polynucleotides involves a washing step in which no ethanol or less than 5% ethanol is used in the buffer that washes the beads to which the hybridized RNA polynucleotides have precipitated so as to separate them from unbound DNA oligonucleotides. A binding buffer that includes the beads is combined with a hybridization solution that includes salt and RNA polynucleotides of bacterial species that are hybridized to DNA oligonucleotides. The binding buffer and the hybridization solution are removed from the aggregated beads. The washing buffer, as is conventionally used, is added to the beads after the binding buffer and the hybridization solution have been removed. The washing buffer is removed from the aggregated beads. An elution buffer is added to the beads after the washing buffer has been removed. The elution buffer which contains the purified hybridized RNA polynucleotides is removed from the aggregated beads.

Anmelder

Firma

mbiomics GmbH

Land

🇩🇪 Deutschland