Oligonukleotidherstellungsverfahren und Nukleosid, Nukleotid oder Oligonukleotid

Details

Veröffentlichungs-Nr.

EP4741411

Anmeldetag

16. Dezember 2016

Veröffentlichung

13. Mai 2026

Rechtsraum

EP

IPC

C07H19/20(HIRAI KUNIHIRO et al.)

Abstract

The present invention provides a production method oligonucleotide, including (1) a step of condensing a nucleoside, nucleotide or oligonucleotide (a) or the like wherein a 5'-hydroxy group is not protected and a nucleoside, nucleotide or oligonucleotide (b) wherein a 5'-hydroxy group is protected in a non-polar solvent to give a reaction solution containing a phosphite triester product (c); (3) a step of oxidizing or sulfurizing the phosphite triester product (c) to give a reaction solution containing an oligonucleotide (d) wherein the 5'-hydroxy group is protected; (4) a step of deprotecting the oligonucleotide (d) to give a reaction solution containing an oligonucleotide (e) wherein the 5'-hydroxy group is not protected; and (6) a step of adding a polar solvent to the reaction solution containing the oligonucleotide (e) and purifying the oligonucleotide (e) by solid-liquid separation or extraction.

Anmelder

Firma

AJINOMOTO CO., INC.

Land

🇯🇵 Japan