Verfahren zur Herstellung einer Trypsin-Ähnlichen Protease in Bacillus

Details

Veröffentlichungs-Nr.

EP4741416

Anmeldetag

11. November 2024

Veröffentlichung

13. Mai 2026

Rechtsraum

EP

IPC

C07K14/32, C12N9/58(PRONUTRIA INC et al.)(DANISCO US INC et al.)(KANEKA CORP et al.)LIOY, PLASMID, 2010

Abstract

The invention relates to a method for producing a trypsin-like protease in Bacillus, the method comprising expressing in a Bacillus host cell a gene coding for a preproenzyme comprising or consisting of (i) a trypsin-like protease having a sequence identity of at least 70% compared to the wild-type trypsin-like protease from the fungus Fusarium oxysporum, and (ii) preferably, a prosequence located upstream of the trypsin-like protease and having at its C-terminus a basic amino acid residue, and (iii) a bacterial secretion signal sequence located upstream of the trypsin-like protease and of the optionally present prosequence, wherein the bacterial secretion signal sequence is capable of directing secretion in a Bacillus host cell to a culture medium, thereby providing a culture medium comprising the mature trypsin-like protease.

Anmelder

Firma

Kerry Group Services International Ltd

Land

🇮🇪 Irland