Rna-Polymerasevariante und Verwendung davon

Details

Veröffentlichungs-Nr.

EP4752219

Anmeldetag

14. Oktober 2025

Veröffentlichung

3. Juni 2026

Rechtsraum

EP

IPC

C12N9/12, C12P19/34

Abstract

The present application provides a class of T7 RNA polymerase variants and the use thereof, wherein the amino acid sequences of the T7 RNA polymerase variants comprise, relative to SEQ ID NO: 1, at least one substitution or deletion at amino acid positions selected from R34, K172, Y178, R386, D388, Q435, N437 or D438. The variants have improved catalytic activity relative to the wild-type T7 RNA polymerase, which can increase the capping rate of mRNA products during in-vitro transcription (co-transcriptional capping). In addition, the present application further provides a method for preparing RNA by using such variants. By adopting the method to prepare RNA molecules, more capped mRNAs can be obtained.

Anmelder

Firma

Nanjing Vazyme Biotech Co., Ltd.

Land

🇨🇳 China

Fachgebiete