Modulares Erfassungskonstrukt und Verfahren zum Nachweis mehrerer Analyten

Details

Veröffentlichungs-Nr.

EP4752232

Anmeldetag

2. Dezember 2024

Veröffentlichung

3. Juni 2026

Rechtsraum

EP

IPC

C12Q1/6816

Abstract

A modular capture construct (100, 900, 1000) for capturing a plurality of analytes (402, 504, 604, 706) of a biological sample (1300, 1408) is provided. The modular capture construct (100, 900, 1000) comprises a plurality of modules (102a, 102b, 102c), and at least a first dye (112) and a second dye (114) arranged on the capture construct (100, 900, 1000) to provide an orientation indication. The modular capture construct further comprises at least a first plurality of capture regions (108a, 108b, 108c, 108d, 108e, 108f), each capture region (108a, 108b, 108c, 108d, 108e, 108f) comprising at least one affinity capture reagent (400, 502, 602, 702, 704) configured to capture one of the analytes (402, 504, 604, 706), wherein each module (102a, 102b, 102c) comprises at least one of the capture regions (108a, 108b, 108c, 108d, 108e, 108f) of the first plurality of capture regions (108a, 108b, 108c, 108d, 108e, 108f). Each module (102a, 102b, 102c) comprises a nucleic acid backbone (201). Further, each of the modules (102a, 102b, 102c) is hybridised to at least another one of the modules (102a, 102b, 102c). In a further aspect, a method for detecting a plurality of analytes of a biological sample by means of the modular capture construct is provided.

Anmelder

Firma

Leica Microsystems CMS GmbH

Land

🇩🇪 Deutschland