Verfahren zum Nachweis von Glykierten Proteinen in Biologischen Proben und Verwendung davon

Details

Veröffentlichungs-Nr.

EP4764499

Anmeldetag

17. Dezember 2024

Veröffentlichung

24. Juni 2026

Rechtsraum

EP

Abstract

The present invention relates to a method of detection of glycated proteins, particularly gHSA, which comprises the steps of:i) providing anthracene boronic acid methacrylate (ABAM): 10-[N-methyl-N-(o-boronobenzyl)aminomethyl]-anthracene-9-methyl methacrylate;ii) preparing a solution of ABAM in a biocompatible buffer with pH in the range of from 6.5 to 8.5 or in in vitro blood plasma sample of a healthy subject;iii) measuring initial fluorescence intensity of ABAM solution from step ii) at the wavelength of from 410 to 520 nm;iv) in vitro mixing of a known amount of ABAM solution from step ii) with a known amount of biological sample;v) measuring fluorescence intensity of the mixture from step iv) at the wavelength of from 410 to 520 nm;vi) determining the fluorescence intensity increases between the initial control fluorescence intensity measured in step iii) and the fluorescence intensity of the mixture of ABAM and biological sample measured in step v);vii) calculating the amount of glycated proteins in the biological sample.The present invention further relates to the use of this method in determination of the total concentrations of glycated proteins, particularly gHSA, in plasma samples and diagnostics for diabetes mellitus.

Anmelder

Firmen

Mendelova Univerzita v Brne
Univerzita Pardubice

Land

🇨🇿 Tschechien