Verfahren zum Nachweis von Exon 8 von Anril in einer Person, Kit, Oligonukleotidesets und deren Verwendungen

Details

Veröffentlichungs-Nr.

EP4768603

Anmeldetag

24. Dezember 2024

Veröffentlichung

1. Juli 2026

Rechtsraum

EP

IPC

C12Q1/6886

Abstract

The present invention relates to a method of detecting the presence of exon 8 of ANRIL (Antisense Non-coding RNA in the INK4 Locus) in a subject, comprising the following steps of:1) Preparation of total RNA from a biological sample of the subject,2) Conversion of RNA molecules into complementary DNA,3) Loop-mediated isothermal amplification using trans-exonic oligonucleotides that specifically produces an amplicon containing exon 8 fragment,4) Addition of Cas12a/RNA guide complexes, wherein RNA guide specifically targets exon 8-containing fragment by base pairing,5) Addition of a reporter DNA probe, and6) Detection of cleavage products,Wherein detection of cleavage products reveals the presence of exon 8 of ANRIL.The present invention also relates to a method for in vitro predicting in vitro the efficacy of a therapy targeting exon 8 of ANRIL in a subject, to a kit for implementing the method and to a set of oligonucleotides.

Anmelder

Firmen

Université de Lorraine
Centre National de la Recherche Scientifique

Land

🇫🇷 Frankreich

Fachgebiete